# Biology

- [Background](/protocol/biology/background.md)
- [Genomics](/protocol/biology/background/genomics.md): 얇고 대충 알아보는 유전체학
- [Chromosome](/protocol/biology/background/genomics/chromosome.md): Chromosome은 X 모양으로 생기지 않았다.
- [Gene](/protocol/biology/background/genomics/gene.md)
- [DNA](/protocol/biology/background/genomics/dna.md)
- [Sequencing](/protocol/biology/background/genomics/sequencing.md)
- [Cell biology](/protocol/biology/background/cell-biology.md)
- [Molecular biology](/protocol/biology/background/molecular-biology.md)
- [pathways](/protocol/biology/background/molecular-biology/pathways.md)
- [Bio-engineering](/protocol/biology/background/molecular-biology/bio-engineering.md)
- [Oncology](/protocol/biology/background/oncology.md)
- [Basic experiments](/protocol/biology/basic-experiments.md)
- [Basic methods](/protocol/biology/basic-experiments/basic-methods.md)
- [Competent cell preparation](/protocol/biology/basic-experiments/basic-methods/competent-cell-preparation.md): Rubidium chloride를 사용한 형질전환용 박테리아 제작
- [General PCR](/protocol/biology/basic-experiments/basic-methods/general-pcr.md): 가장 기본적이면서도 어려운 실험
- [Overlap extension PCR](/protocol/biology/basic-experiments/basic-methods/overlap-extension-pcr.md): 짧은 fragment끼리 빠르게 연결해서 긴 fragment로 만드는 방법
- [Gel purification](/protocol/biology/basic-experiments/basic-methods/gel-purification.md): DNA fragment를 size selection하기 위한 방법
- [Cloning](/protocol/biology/basic-experiments/cloning.md)
- [Restriction enzyme](/protocol/biology/basic-experiments/cloning/restriction-enzyme.md)
- [PNK annealing](/protocol/biology/basic-experiments/cloning/pnk-annealing.md): Oligo의 5' 말단에 phosphate를 붙여주면서 상보적인 서열을 붙여주는 과정
- [T4 ligation](/protocol/biology/basic-experiments/cloning/t4-ligation.md)
- [Golden gate cloning](/protocol/biology/basic-experiments/cloning/golden-gate-cloning.md)
- [Gibson assembly](/protocol/biology/basic-experiments/cloning/gibson-assembly.md)
- [Culture](/protocol/biology/basic-experiments/culture.md)
- [Bacteria](/protocol/biology/basic-experiments/culture/bacteria.md)
- [Mammalian cell](/protocol/biology/basic-experiments/culture/mammalian-cell.md)
- [Cell lines](/protocol/biology/basic-experiments/culture/mammalian-cell/cell-lines.md): 다양한 cell line들에 대한 배양 경험을 정리한 기록
- [Culture media](/protocol/biology/basic-experiments/culture/mammalian-cell/culture-media.md): 기본 배양액과 기타 첨가물
- [Delivery](/protocol/biology/basic-experiments/delivery.md)
- [Transformation](/protocol/biology/basic-experiments/delivery/transformation.md): E. Coli competent cell에 plasmid DNA를 전달하는 방법
- [Transfection](/protocol/biology/basic-experiments/delivery/transfection.md)
- [Transduction](/protocol/biology/basic-experiments/delivery/transduction.md)
- [Transposition](/protocol/biology/basic-experiments/delivery/transposition.md)
- [Sequencing](/protocol/biology/basic-experiments/sequencing.md)
- [Sanger](/protocol/biology/basic-experiments/sequencing/sanger.md)
- [NGS](/protocol/biology/basic-experiments/sequencing/ngs.md)
- [Long-read sequencing](/protocol/biology/basic-experiments/sequencing/long-read-sequencing.md)
- [Page 3](/protocol/biology/basic-experiments/page-3.md)
- [Equipment guide](/protocol/biology/equipment-guide.md)
- [Genome editing](/protocol/biology/genome-editing.md): 유전자의 원하는 위치에 특정 변이를 일으키는 방법
- [CRISPR-Cas system](/protocol/biology/genome-editing/crispr-cas-system.md): 박테리아의 면역 체계에서 유전자 교정 도구가 되기까지
- [Cas nuclease](/protocol/biology/genome-editing/cas-nuclease.md)
- [Base editing](/protocol/biology/genome-editing/base-editing.md)
- [Prime editing](/protocol/biology/genome-editing/prime-editing.md)
- [Mobile element systems](/protocol/biology/genome-editing/mobile-element-systems.md)
- [Transposase](/protocol/biology/genome-editing/mobile-element-systems/transposase.md)
- [Integrase](/protocol/biology/genome-editing/mobile-element-systems/integrase.md)
- [Recombinase](/protocol/biology/genome-editing/mobile-element-systems/recombinase.md)
- [mtDNA editing](/protocol/biology/genome-editing/mtdna-editing.md): Mitochondria genome editing, 최근 활발하게 연구되고 있는 분야
- [Analysis](/protocol/biology/genome-editing/analysis.md)
- [Useful tools](/protocol/biology/useful-tools.md)
- [CRISPR prediction tools](/protocol/biology/useful-tools/crispr-prediction-tools.md)
- [Codon optimization](/protocol/biology/useful-tools/codon-optimization.md): GenSmart Codon Optimization (GenScript)
- [Protein structure](/protocol/biology/useful-tools/protein-structure.md)
- [PyMol](/protocol/biology/useful-tools/protein-structure/pymol.md)
- [Protein 3D image](/protocol/biology/useful-tools/protein-structure/protein-3d-image.md): 3D protein imaging: PDB 파일을 쉽게 시각화 할 수 있는 웹 서비스
- [Research note](/protocol/biology/research-note.md): 실제 진행했던 연구 사례 모음
- [CRISPR screening](/protocol/biology/research-note/crispr-screening.md)
- [RNP transfection](/protocol/biology/research-note/rnp-transfection.md)
- [Page 7](/protocol/biology/research-note/page-7.md)