# Cloning

- [Restriction enzyme](/protocol/biology/basic-experiments/cloning/restriction-enzyme.md)
- [PNK annealing](/protocol/biology/basic-experiments/cloning/pnk-annealing.md): Oligo의 5' 말단에 phosphate를 붙여주면서 상보적인 서열을 붙여주는 과정
- [T4 ligation](/protocol/biology/basic-experiments/cloning/t4-ligation.md)
- [Golden gate cloning](/protocol/biology/basic-experiments/cloning/golden-gate-cloning.md)
- [Gibson assembly](/protocol/biology/basic-experiments/cloning/gibson-assembly.md)